Biphasic nanofibrous scaffolds based on collagen and PLC for controlled release LL‐37 in guided bone regeneration. Issue 7 (13th September 2021)

• Record Type: Journal Article
• Authors: Prakobkarn, Jeeranan; Makeudom, Anupong; Jenvoraphot, Thannaphat; Supanchart, Chayarop; Krisanaprakornkit, Suttichai; Punyodom, Winita; Daranarong, Donraporn
• Is Part Of: Journal of applied polymer science. Volume 139:Issue 7(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-09-13
• Subjects: applications -- biodegradable -- biomaterials -- biomedical applications -- membranes; Polymers -- Periodicals; Polymerization -- Periodicals; 668.9
• DOI: 10.1002/app.51629
• Languages: English
• ISSNs: 0021-8995

Abstract

Abstract: This is a feasibility study on the ability of biodegradable collagen coated with poly(L‐lactide‐ co ‐ ε ‐caprolactone) (PLC) to deliver the antimicrobial peptide, LL‐37, for usage as a barrier membrane in guided bone regeneration. In this study, a nanofibrous PLC is fabricated using the electrospinning technique to establish the controlled release of the LL‐37, which adsorbed onto collagen scaffold (LL/Col), in order to produce a LL/Col/PLC membrane. The in vitro released profiles of the LL‐37 obtained from the LL/Col/PLC membranes are studied in phosphate buffer saline and are compared with unfabricated LL/Col membranes. According to the results, the LL/Col/PLC membrane is capable of delivering LL‐37 in a sustained release in amounts that has no cytotoxicity over a period of 14 days. An assessment of the biocompatibility of the LL/Col/PLC membrane indicate that it can enhance the proliferation of mouse fibroblasts (L929) and human monocytes that are known to be a key regulator in the bone healing process. Moreover, the surface topography of the LL/Col/PLC membrane mimics the extracellular matrix by establishing cell attachment and proliferation. These findings indicate that the LL/Col/PLC membrane developed in this study can sustain the release of LL‐37 in clinically relevant doses, which could then further enhance bone regeneration. Abstract : A biphasic biodegradable membrane as LL/Col/PLC membrane for usage as a barrier membrane in guided bone regeneration can enhance in sustained release of antimicrobial peptide from nanofibrous scaffolds in clinically relevant doses. Moreover, LL/Col/PLC membrane was non‐toxic and could enhance the proliferation of human monocytes that are known to be a key regulator in the bone healing process.