Adsorption‐Inhibition of Clathrate Hydrates by Self‐Assembled Nanostructures. Issue 21 (6th September 2021)

• Record Type: Journal Article
• Authors: Soussana, Tamar Nicole; Weissman, Haim; Rybtchinski, Boris; Drori, Ran
• Is Part Of: Chemphyschem. Volume 22:Issue 21(2021)
• Page Start: 2182
• Page End: 2189
• Publication Date: 2021-09-06
• Subjects: antifreeze proteins -- crystal growth -- microfluidics -- safranine -- self-assembly; Chemistry, Physical and theoretical -- Periodicals; 541.05
• DOI: 10.1002/cphc.202100463
• Languages: English
• ISSNs: 1439-4235

Abstract

Abstract: The mechanism by which safranine O (SFO), an ice growth inhibitor, halts the growth of single crystal tetrahydrofuran (THF) clathrate hydrates was explored using microfluidics coupled with cold stages and fluorescence microscopy. THF hydrates grown in SFO solutions exhibited morphology changes and were shaped as truncated octahedrons or hexagons. Fluorescence microscopy and microfluidics demonstrated that SFO binds to the surface of THF hydrates on specific crystal planes. Cryo‐TEM experiments of aqueous solutions containing millimolar concentrations of SFO exhibited the formation of bilayered lamellae with an average thickness of 4.2±0.2 nm covering several μm 2 . Altogether, these results indicate that SFO forms supramolecular lamellae in solution, which might bind to the surface of the hydrate and inhibit further growth. As an ice and hydrate inhibitor, SFO may bind to the surface of these crystals via ordered water molecules near its amine and methyl groups, similar to some antifreeze proteins. Abstract : Microfluidics and fluorescence microscopy revealed that safranine O binds to clathrate hydrates surfaces and inhibits their growth. Cryo‐TEM imagery exhibited the formation of lamella films in safranine solutions. A molecular model of safranine films suggests structural similarities to antifreeze proteins that also bind to ice and hydrates.