Turn on chemiluminescence-based probes for monitoring tyrosinase activity in conjunction with biological thiols. Issue 86 (14th October 2021)

• Record Type: Journal Article
• Authors: Shelef, Omri; Sedgwick, Adam C.; Pozzi, Sabina; Green, Ori; Satchi-Fainaro, Ronit; Shabat, Doron; Sessler, Jonathan L.
• Is Part Of: Chemical communications. Volume 57:Issue 86(2021)
• Page Start: 11386
• Page End: 11389
• Publication Date: 2021-10-14
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc05217a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We report a chemiluminescent probe that permits the paired detection of tyrosinase (Tyr) and biological thiols. The Tyr-formed benzoquinone intermediate reacts with GSH and produces an enhanced chemiluminescence response. Abstract : We report a chemiluminescent probe (CLPT1 ) that permits the paired detection of tyrosinase (Tyr) and biological thiols. Tyr only leads to a poor chemiluminescence response, a finding ascribed to the formation of a stable o -benzoquinone intermediate. The addition of glutathione (GSH), or ascorbate to the o -benzoquinone intermediate results in thiol conjugation or reduction to this intermediate, respectively. This produces a strong chemiluminescence response. Thiol co-dependence was demonstrated in live cells using the cell permeable analogue, CLPT3 . The present chemiluminescence-based strategy allows the concurrent detection of tyrosinase activity and biological thiols.