Late-stage modification of peptides and proteins at cysteine with diaryliodonium salts. Issue 42 (12th October 2021)

• Record Type: Journal Article
• Authors: Byrne, Stephen A.; Bedding, Max J.; Corcilius, Leo; Ford, Daniel J.; Zhong, Yichen; Franck, Charlotte; Larance, Mark; Mackay, Joel P.; Payne, Richard J.
• Is Part Of: Chemical science. Volume 12:Issue 42(2021)
• Page Start: 14159
• Page End: 14166
• Publication Date: 2021-10-12
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1sc03127a
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : New operationally simple platform for the chemoselective arylation of cysteine in peptides and proteins to access a variety of high value bioconjugates. Abstract : The modification of peptides and proteins has emerged as a powerful means to efficiently prepare high value bioconjugates for a range of applications in chemical biology and for the development of next-generation therapeutics. Herein, we report a novel method for the chemoselective late-stage modification of peptides and proteins at cysteine in aqueous buffer with suitably functionalised diaryliodonium salts, furnishing stable thioether-linked synthetic conjugates. The power of this new platform is showcased through the late-stage modification of the affibody zEGFR and the histone protein H2A.