Pseudomonas schmalbachii sp. nov., isolated from the gut of a millipede (Trigoniulus corallinus) from a coconut tree. Issue 10 (27th October 2021)

• Record Type: Journal Article
• Authors: Shelomi, Matan; Chen, Wen-Ming; Chen, Hsin-Kuang; Lee, Hsin-Ying; Young, Chiu-Chung; Lin, Shih-Yao; Liaw, Shwu-Jen
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 10(2021)
• Publication Date: 2021-10-27
• Subjects: new species -- Pseudomonas -- taxonomic description; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005101
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : During an investigation of microbes associated with arthropods living in decaying coconut trees, a Pseudomonas isolate, Milli4 T, was cultured from the digestive tract of the common Asian millipede, Trigoniulus corallinus . Sequence analysis of 16S rRNA and rpoB genes found that Milli4 T was closely related but not identical to Pseudomonas panipatensis Esp-1 T, Pseudomonas knackmussi B13 T and Pseudomonas humi CCA1 T . Whole genome sequencing suggested that this isolate represents a new species, with average nucleotide identity (OrthoANIu) values of around 83.9–87.7% with its closest relatives. Genome-to-genome distance calculations between Milli4 T and its closest relatives also suggested they are distinct species. The genomic DNA G+C content of Milli4 T was approximately 65.0 mol%. Phenotypic and chemotaxonomic characterization and fatty acid methyl ester analysis was performed on Milli4 T and its related type strains. Based on these data, the new species Pseudomonas schmalbachii sp. nov. is proposed, and the type strain is Milli4 T (=BCRC 81294 T =JCM 34414 T =CIP 111980 T ).