Description of antibiotic-producing novel bacteria Paraburkholderia antibiotica sp. nov. and Paraburkholderia polaris sp. nov. Issue 10 (25th October 2021)

• Record Type: Journal Article
• Authors: Dahal, Ram Hari; Kim, Jungmin; Chaudhary, Dhiraj Kumar; Kim, Dong-Uk; Kim, Jaisoo
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 10(2021)
• Publication Date: 2021-10-25
• Subjects: antimicrobial resistance -- antimicrobial agents -- Arctic soil -- psychrotolerant -- forest soil; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005060
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Two white colony-forming, Gram-stain-negative, non-sporulating and motile bacteria, designated G-4-1-8 T and RP-4-7 T, were isolated from forest soil and Arctic soil, respectively. Both strains showed antimicrobial activity against Gram-negative pathogens ( Pseudomonas aeruginosa and Escherichia coli ) and could grow at a pH range of pH 4.0–11.0 (optimum, pH 7.0–9.0). Phylogenetic analyses based on their 16S rRNA gene sequences indicated that strains G-4-1-8 T and RP-4-7 T formed a lineage within the family Burkholderiaceae and were clustered as members of the genus Paraburkholderia . Strain G-4-1-8 T showed the highest 16S rRNA sequence similarity to Paraburkholderia monticola JC2948 T (98.1 %), while strain RP-4-7 T showed the highest similarity to Paraburkholderia metrosideri DNBP6-1 T (98.8 %). The only respiratory quinone in both strains was ubiquinone Q-8. Their principal cellular fatty acids were C16 : 0, cyclo-C17 : 0, summed feature 3 (iso-C15 :0 2-OH and/or C16 :1 ω 7 c ) and summed feature 8 (C18 : 1 ω 7 c and/or C18 : 1 ω 6 c ). Their major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unidentified aminophospholipid. The DNA G+C content of strains G-4-1-8 T and RP-4-7 T were 63.7 and 61.3 mol%, respectively, while their genome lengths were 7.44 and 9.67 Mb, respectively. The genomes of both strains showed at least 12 putative biosynthetic gene clusters. The average nucleotide identity and in silico DNA–DNA hybridization relatedness values between both strains and most closely related Paraburkholderia species were below the species threshold values. Based on a polyphasic study, these isolated strains represent novel species belonging to the genus Paraburkholderia, for which the names Paraburkholderia antibiotica sp. nov. (G-4-1-8 T = KACC 21617 T =NBRC 114603 T ) and Paraburkholderia polaris sp. nov. (RP-4-7 T =KACC 21621 T =NBRC 114605 T ) are proposed.