Treponema peruense sp. nov., a commensal spirochaete isolated from human faeces. Issue 10 (21st October 2021)

• Record Type: Journal Article
• Authors: Belkhou, Claire; Tadeo, Raul Tito; Bacigalupe, Rodrigo; Valles-Colomer, Mireia; Chaffron, Samuel; Joossens, Marie; Obregon, Alexandra; Marín Reyes, Luis; Trujillo, Omar; Huys, Geert R. B.; Raes, Jeroen
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 10(2021)
• Publication Date: 2021-10-21
• Subjects: faeces -- gut -- human -- microbiome -- spirochaete -- Treponema; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005050
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, obligatory anaerobic spirochaete (RCC2812 T ) was isolated from a faecal sample obtained from an individual residing in a remote Amazonian community in Peru. The bacterium showed highest 16S rRNA gene sequence similarity to the pig intestinal spirochete Treponema succinifaciens (89.48 %). Average nucleotide identity values between strain RCC2812 T and all available Treponema genomes from validated type strains were all <73 %, thus clearly lower than the species delineation threshold. The DNA G+C content of RCC2812 T was 41.24 mol%. Phenotypic characterization using the API-ZYM and API 20A systems confirmed the divergent position of this bacterium within the genus Treponema . Strain RCC2812 T could be differentiated from the phylogenetically most closely related T. succinifaciens by the presence of alkaline phosphatase and α -glucosidase activities. Unlike T. succinifaciens, strain RCC2812 T grew equally well with or without serum. Strain RCC2812 T is the first commensal Treponema isolated from the human faecal microbiota of remote populations, and based on the collected data represents a novel Treponema species for which the name Treponema peruense sp. nov. is proposed. The type strain is RCC2812 T (=LMG 31794 T =CIP 111910 T ).