Antifungal weapons of Lysobacter, a mighty biocontrol agent. (21st July 2021)

• Record Type: Journal Article
• Authors: Lin, Long; Xu, Kangwen; Shen, Danyu; Chou, Shan‐Ho; Gomelsky, Mark; Qian, Guoliang
• Other Names: Malone Jacob guestEditor.; de Jonge Ronnie guestEditor.; Eberl Leo guestEditor.; Bernal Patricia guestEditor.; Lepek Viviana guestEditor.
• Is Part Of: Environmental microbiology. Volume 23:Number 10(2021)
• Page Start: 5704
• Page End: 5715
• Publication Date: 2021-07-21
• Subjects: Microbial ecology -- Periodicals; Environmental Microbiology -- Periodicals; 579.17
• DOI: 10.1111/1462-2920.15674
• Languages: English
• ISSNs: 1462-2912

Abstract

Summary: Bacteria interact with fungi in a variety of ways to inhibit fungal growth, while the underlying mechanisms remain only partially characterized. The plant‐beneficial Bacillus and Pseudomonas species are well‐known antifungal biocontrol agents, whereas Lysobacter are far less studied. Members of Lysobacter are easy to grow in fermenters and are safe to humans, animals and plants. These environmentally ubiquitous bacteria use a diverse arsenal of weapons to prey on other microorganisms, including fungi and oomycetes. The small molecular toxins secreted by Lysobacter represent long‐range weapons effective against filamentous fungi. The secreted hydrolytic enzymes act as intermediate‐range weapons against non‐filamentous fungi. The contact‐dependent killing devices are proposed to work as short‐range weapons. We describe here the structure, biosynthetic pathway, action mode and applications of one of the best‐characterized long‐range weapons, the heat‐stable antifungal factor (HSAF) produced by Lysobacter enzymogenes . We discuss how the flagellar type III secretion system has evolved into an enzyme secretion machine for the intermediate‐range antifungal weapons. We highlight an intricate mechanism coordinating the production of the long‐range weapon, HSAF and the proposed contact‐dependent killing device, type VI secretion system. We also overview the regulatory mechanisms of HSAF production involving specific transcription factors and the bacterial second messenger c‐di‐GMP.