A new comprehensive annotation of leucine‐rich repeat‐containing receptors in rice. (2nd September 2021)

• Record Type: Journal Article
• Authors: Gottin, Céline; Dievart, Anne; Summo, Marilyne; Droc, Gaëtan; Périn, Christophe; Ranwez, Vincent; Chantret, Nathalie
• Is Part Of: Plant journal. Volume 108:Number 2(2021)
• Page Start: 492
• Page End: 508
• Publication Date: 2021-09-02
• Subjects: LRR‐receptor‐like kinase -- LRR‐receptor‐like protein -- nucleotide‐binding LRR receptor -- annotation curation -- pseudogenes -- Oryza sativa -- disease resistance gene; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.15456
• Languages: English
• ISSNs: 0960-7412

Abstract

SUMMARY: Oryza sativa (rice) plays an essential food security role for more than half of the world's population. Obtaining crops with high levels of disease resistance is a major challenge for breeders, especially today, given the urgent need for agriculture to be more sustainable. Plant resistance genes are mainly encoded by three large leucine‐rich repeat (LRR)‐containing receptor (LRR‐CR) families: the LRR‐receptor‐like kinase (LRR‐RLK), LRR‐receptor‐like protein (LRR‐RLP) and nucleotide‐binding LRR receptor (NLR). Using lrrprofiler, a pipeline that we developed to annotate and classify these proteins, we compared three publicly available annotations of the rice Nipponbare reference genome. The extended discrepancies that we observed for LRR‐CR gene models led us to perform an in‐depth manual curation of their annotations while paying special attention to nonsense mutations. We then transferred this manually curated annotation to Kitaake, a cultivar that is closely related to Nipponbare, using an optimized strategy. Here, we discuss the breakthrough achieved by manual curation when comparing genomes and, in addition to 'functional' and 'structural' annotations, we propose that the community adopts this approach, which we call 'comprehensive' annotation. The resulting data are crucial for further studies on the natural variability and evolution of LRR‐CR genes in order to promote their use in breeding future resilient varieties. Significance Statement: The automatic structural annotation of genes evolving through an active birth‐and‐death model, such as LRR‐CRs, is often inconsistent because numerous paralogs accumulate nonsense mutations in their coding sequences. We propose a consensus method of annotating these genes while retaining all sequence fragments that are clearly derived from their ancestral coding sequences. They are then tagged as non‐canonical or canonical, respectively, depending on the presence or absence of nonsense mutations.