Preparative production of an enantiomeric pair by engineered polyketide synthases. Issue 70 (11th August 2021)

• Record Type: Journal Article
• Authors: Miyazawa, Takeshi; Fitzgerald, Brendan J.; Keatinge-Clay, Adrian T.
• Is Part Of: Chemical communications. Volume 57:Issue 70(2021)
• Page Start: 8762
• Page End: 8765
• Publication Date: 2021-08-11
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc03073f
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Modules from the pikromycin synthase were recombined into engineered synthases that furnish an enantiomeric pair of 2-stereocenter triketide lactones at >99% ee with yields up to 0.39 g per liter of E. coli K207-3 in shake flasks. Abstract : Using the updated module boundary of polyketide assembly lines, modules from the pikromycin synthase were recombined into engineered synthases that furnish an enantiomeric pair of 2-stereocenter triketide lactones at >99% ee with yields up to 0.39 g per liter of E. coli K207-3 in shake flasks.