A reference scale of cucurbit[7]uril binding affinities. Issue 39 (10th August 2021)

• Record Type: Journal Article
• Authors: Alnajjar, Mohammad A.; Nau, Werner M.; Hennig, Andreas
• Is Part Of: Organic & biomolecular chemistry. Volume 19:Issue 39(2021)
• Page Start: 8521
• Page End: 8529
• Publication Date: 2021-08-10
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/d1ob01304a
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : The binding of the macrocyclic host cucurbit[7]uril with selected guest molecules has been re-investigated to cover a large range in affinities (millimolar to femtomolar) and to set up a reference scale for future binding constant determinations. Abstract : The accurate determination of ultra-high binding affinities in supramolecular host–guest chemistry is a challenging endeavour because direct binding titrations are generally limited to affinities <10 6 M −1 due to sensitivity constraints of common titration methods. To determine higher affinities, competitive titrations are usually performed, in which one compound with a well established binding affinity serves as a reference. Herein, we propose a reference scale for such competitive titrations with the host cucurbit[7]uril (CB7) comprising binding affinities in the range from 10 3 to 10 15 M −1 . The suggested reference compounds are commercially available and will aid in the future determination of CB7 binding affinities for stimuli-responsive host–guest systems.