What's a SNP between friends: The lineage of Clostridioides difficile R20291 can effect research outcomes. (October 2021)

• Record Type: Journal Article
• Authors: Monteford, Jorge; Bilverstone, Terry W.; Ingle, Patrick; Philip, Sheryl; Kuehne, Sarah A.; Minton, Nigel P.
• Is Part Of: Anaerobe. Volume 71(2021)
• Publication Date: 2021-10
• Subjects: Clostridioides difficile R20291 -- Motility -- Biofilm -- Toxin production -- Conjugation -- Genomic variation; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2021.102422
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: Clostridioides difficile R20291 is the most studied PCR-Ribotype 027 isolate. The two predominant lineages of this hypervirulent strain, however, exhibit substantive phenotypic differences and possess genomes that differ by a small number of nucleotide changes. It is important that the source of R20291 is taken into account in research outcomes. Highlights: Two phenotypically distinct lineages of C. difficile R20291 are in circulation. Affected are gene transfer, motility, sporulation, biofilm and toxin production. The differences are due to a small number of single nucleotide genomic changes. These findings underline the importance of appropriately maintaining stock cultures. The source of R20291 should be stated in any investigation.