Fluorescent glutamine and asparagine as promising probes for chemical biology. Issue 35 (26th August 2021)

• Record Type: Journal Article
• Authors: Bajaj, Kiran; Pidiyara, Karishma; Khan, Shahid; Jha, Prabhat N.; Sakhuja, Rajeev; Kumar, Dalip
• Is Part Of: Organic & biomolecular chemistry. Volume 19:Issue 35(2021)
• Page Start: 7695
• Page End: 7700
• Publication Date: 2021-08-26
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/d1ob01029h
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Fluorescent glutamine and asparagine with excellent photophysical properties are showcased. Cell permeation of these fluorescent amino acids proved their potential as fluorescent probes for chemical biology. Abstract : Fluorescent probes have become valuable tools in chemical biology, providing interesting inferences for unfolding the complexities of natural biochemical processes. In this study, we report the synthesis and characterization of fluorescent labelled glutamine (Gln) and asparagine (Asn) derivatives via traceless Staudinger ligation, which exhibited high fluorescence quantum yields, excellent photostabilities and emission of blue fluorescence in the visible region. The successful permeation of these fluorescent amino acids into cellular components proved their potential as fluorescent probes for chemical biology.