A new G-triplex-based strategy for sensitivity enhancement of the detection of endonuclease activity and inhibition. Issue 45 (19th August 2021)

• Record Type: Journal Article
• Authors: Gao, Congcong; Che, Baoquan; Dai, Hong
• Is Part Of: RSC advances. Volume 11:Issue 45(2021)
• Page Start: 28008
• Page End: 28013
• Publication Date: 2021-08-19
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra04203c
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A new G-triplex-based probe was developed for detecting EcoRI activity and inhibition. The probe showed good selectivity towards EcoRI. The assay was colorimetric and can be monitored by the naked eye. Abstract : EcoRI is an important biomacromolecule in live cells and protects bacterial cells against foreign DNA. In this work, we developed a simple and convenient G-triplex (G3) (5′-TGGGAAGGGAGGGAATTCCCT-3′)-based colorimetric assay for the rapid and selective detection of EcoRI activity and inhibition. The sequence specifically responds to EcoRI in the presence of K + and hemin to form a G-triplex/hemin complex. Taking advantage of G-triplex, EcoRI activity was investigated under the optimized conditions. The absorption intensity ratio displayed a linear relationship against the concentration of EcoRI in the range 0 to 100 U mL −1, and the detection limit was 5.7 U mL −1 . Furthermore, G3 showed good selectivity, and the ability to be used to screen for EcoRI inhibitors, indicating its potential in detection and analysis applications.