Recent advances in biological nanopores for nanopore sequencing, sensing and comparison of functional variations in MspA mutants. Issue 46 (31st August 2021)

• Record Type: Journal Article
• Authors: Bhatti, Huma; Jawed, Rohil; Ali, Irshad; Iqbal, Khurshid; Han, Yan; Lu, Zuhong; Liu, Quanjun
• Is Part Of: RSC advances. Volume 11:Issue 46(2021)
• Page Start: 28996
• Page End: 29014
• Publication Date: 2021-08-31
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d1ra02364k
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Future of nanopore DNA sequencing: schematic illustration shows the future of nanopore DNA sequencing by using a customized biological nanopore with appropriate fabrication. Abstract : Biological nanopores are revolutionizing human health by the great myriad of detection and diagnostic skills. Their nano-confined area and ingenious shape are suitable to investigate a diverse range of molecules that were difficult to identify with the previous techniques. Additionally, high throughput and label-free detection of target analytes instigated the exploration of new bacterial channel proteins such as Fragaceatoxin C (FraC), Cytolysin A (ClyA), Ferric hydroxamate uptake component A (FhuA) and Curli specific gene G (CsgG) along with the former ones, like α-hemolysin (αHL), Mycobacterium smegmatis porin A (MspA), aerolysin, bacteriophage phi 29 and Outer membrane porin G (OmpG). Herein, we discuss some well-known biological nanopores but emphasize on MspA and compare the effects of site-directed mutagenesis on the detection ability of its mutants in view of the surface charge distribution, voltage threshold and pore–analyte interaction. We also discuss illustrious and latest advances in biological nanopores for past 2–3 years due to limited space. Last but not the least, we elucidate our perspective for selecting a biological nanopore and propose some future directions to design a customized nanopore that would be suitable for DNA sequencing and sensing of other nontrivial molecules in question.