CRISPR/Cas12a-mediated liposome-amplified strategy for the photoelectrochemical detection of nucleic acid. Issue 71 (17th August 2021)

• Record Type: Journal Article
• Authors: Gong, Hexiang; Wu, Yulin; Zeng, Ruijin; Zeng, Yongyi; Liu, Xiaolong; Tang, Dianping
• Is Part Of: Chemical communications. Volume 57:Issue 71(2021)
• Page Start: 8977
• Page End: 8980
• Publication Date: 2021-08-17
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc03743a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : This study reports a photoelectrochemical biosensor for dopamine-loaded liposome-encoded magnetic beads cleaved by CRISPR/Cas 12a system for the quantification of human papilloma virus-related DNA using neodymium-doped BiOBr nanosheets. Abstract : This study reports a photoelectrochemical biosensor for dopamine-loaded liposome-encoded magnetic beads cleaved by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas 12a system for the quantification of human papilloma virus (HPV)-related DNA using neodymium-doped BiOBr nanosheets (Nd-BiOBr) as a photoactive matrix. Magnetic beads and dopamine-loaded liposomes are covalently attached to the both ends of ssDNA to construct dumbbell-shaped dopamine-loaded liposome-encoded magnetic bead (DLL-MB) probes. When the guide RNA binds to the target HPV-16, the ssDNA will be cleaved by Cas12a, thereby degrading the double dumbbell probes. After magnetic separation, the dissolved DLLs are treated with Triton X-100 to release the dopamine (as an electron donor), which was then detected by an amplified photocurrent using the Nd-BiOBr-based photoelectrode.