Image fusion of IR and optical microscopy for mapping of biomolecules in tissue. Issue 19 (9th September 2021)

• Record Type: Journal Article
• Authors: Al Jedani, Safaa; Whitley, Conor A.; Ellis, Barnaby G.; Triantafyllou, Asterios; Smith, Caroline I.; Gunning, Philip J.; Gardner, Peter; Risk, Janet M.; Weightman, Peter; Barrett, Steve D.
• Is Part Of: Analyst. Volume 146:Issue 19(2021)
• Page Start: 5848
• Page End: 5854
• Publication Date: 2021-09-09
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d1an01161h
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : We demonstrate image fusion of H&E stains (left) with FTIR images (centre) to create fused images (right) that have both the spatial resolution of the H&E and the chemical information of the FTIR. Abstract : It is shown that a pixel-level image fusion technique can produce images that combine the spatial resolution of optical microscopy images of haematoxylin and eosin (H&E) stained tissue with the chemical information in Fourier transform infrared (FTIR) images. The fused images show minimal distortion and the higher spatial resolution of the H&E images overcomes the diffraction limit on the spatial resolution of the FTIR images. A consideration of the FTIR spectra of nucleic acids and collagen can explain the changes in contrast between non-cancerous oral epithelium and underlying stroma within fused images formed by combining an H&E stain of oral tissue with FTIR images of the tissue obtained at a number of wavenumbers.