Linkage-selective derivatization for glycosylation site- and glycoform-specific characterization of sialic acid isomers using mass spectrometry. Issue 75 (31st August 2021)

• Record Type: Journal Article
• Authors: Peng, Ye; Gu, Bing; Sun, Zhenyu; Li, Yueyue; Zhang, Ying; Lu, Haojie
• Is Part Of: Chemical communications. Volume 57:Issue 75(2021)
• Page Start: 9590
• Page End: 9593
• Publication Date: 2021-08-31
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc04142h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A linkage-selective stable isotope labelling approach for the MS-based differentiation and relative quantification of sialyl-linkage isomers of N -glycopeptide is reported. Abstract : Here, we developed a linkage-selective derivatization approach for the differentiation and relative quantification of α-2, 3- and α-2, 6-linked sialic acids in a site- and glycoform-specific manner. Linkage-selective derivatization with isotope molecules discriminates the isomeric glycopeptides easily using MS and provided a tool for biomarker discovery using the quantitative analysis of isomeric glycopeptides.