Cell-free riboswitches. Issue 5 (4th August 2021)

• Record Type: Journal Article
• Authors: Tabuchi, Takeshi; Yokobayashi, Yohei
• Is Part Of: RSC chemical biology. Volume 2:Issue 5(2021)
• Page Start: 1430
• Page End: 1440
• Publication Date: 2021-08-04
• Subjects: 572
• DOI: 10.1039/d1cb00138h
• Languages: English
• ISSNs: 2633-0679

Abstract

Abstract : Synthetic riboswitches can be used as chemical gene switches in cell-free protein synthesis systems. We provide a current perspective on the state of cell-free riboswitch technologies and their future directions. Abstract : The emerging community of cell-free synthetic biology aspires to build complex biochemical and genetic systems with functions that mimic or even exceed those in living cells. To achieve such functions, cell-free systems must be able to sense and respond to the complex chemical signals within and outside the system. Cell-free riboswitches can detect chemical signals via RNA–ligand interaction and respond by regulating protein synthesis in cell-free protein synthesis systems. In this article, we review synthetic cell-free riboswitches that function in both prokaryotic and eukaryotic cell-free systems reported to date to provide a current perspective on the state of cell-free riboswitch technologies and their limitations.