RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells. Issue 10 (6th September 2018)

• Record Type: Journal Article
• Authors: Tian, Li; Chou, Hong-Li; Zhang, Laining; Hwang, Seon-Kap; Starkenburg, Shawn R.; Doroshenk, Kelly A.; Kumamaru, Toshihiro; Okita, Thomas W.
• Is Part Of: The Plant Cell. Volume 30:Issue 10(2018)
• Page Start: 2529
• Page End: 2552
• Publication Date: 2018-09-06
• DOI: 10.1105/tpc.18.00321
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract : Assays of three key mutations reveal that RBP-P is indispensable for the localization of glutelin and prolamine mRNAs in rice endosperm cells. Abstract: In developing rice ( Oryza sativa ) endosperm, mRNAs of the major storage proteins, glutelin and prolamine, are transported and anchored to distinct subdomains of the cortical endoplasmic reticulum. RNA binding protein RBP-P binds to both glutelin and prolamine mRNAs, suggesting a role in some aspect of their RNA metabolism. Here, we show that rice lines expressing mutant RBP-P mislocalize both glutelin and prolamine mRNAs. Different mutant RBP-P proteins exhibited varying degrees of reduced RNA binding and/or protein-protein interaction properties, which may account for the mislocalization of storage protein RNAs. In addition, partial loss of RBP-P function conferred a broad phenotypic variation ranging from dwarfism, chlorophyll deficiency, and sterility to late flowering and low spikelet fertility. Transcriptome analysis highlighted the essential role of RBP-P in regulating storage protein genes and several essential biological processes during grain development. Overall, our data demonstrate the significant roles of RBP-P in glutelin and prolamine mRNA localization and in the regulation of genes important for plant growth and development through its RNA binding activity and cooperative regulation with interacting proteins.