Consequences of the different order of immunoglobulin gene rearrangements in swine. (January 2022)

• Record Type: Journal Article
• Authors: Sinkora, Marek; Stepanova, Katerina; Sinkorova, Jana
• Is Part Of: Developmental and comparative immunology. Volume 126(2022)
• Publication Date: 2022-01
• Subjects: Porcine immune system -- B cell development -- Immunoglobulin rearrangement -- B cell receptors -- Cell differentiation -- Other animals; BCR B cell receptor -- BM bone marrow -- IgH heavy chain -- IgL light chain -- KDE kappa deleting element (for all other species) or recombining sequence (mice) -- SLC surrogate light chain -- VDJ or VDJH rearrangement of V to D to J gene segments for immunoglobulin heavy chain -- VJ or VJL rearrangement of V to J gene segments for immunoglobulin light chain; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2021.104196
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Swine use a reverse order of immunoglobulin chain rearrangement compared to humans and mice, and this altered and modified order should have measurable consequences. Here we perform new and defining experiments with developing and mature B cells, characterizing the B cell populations that do not exist in other species. First, we have finally confirmed that light chains κ and λ are rearranged and expressed on the surface before any heavy chain rearrangements using western-blot. And second, we have analyzed a pool of mature B cells on the single-cell level to demonstrate that many κ + mature B cells carry λ transcripts. According to these findings, we believe that there may be more groups of mammals; one of which uses a pre-BCR-driven developmental pathway for B cell generation (like mice and humans), the second group uses a pre-BCR-independent one (like swine), and some may be even intermediate. Graphical abstract: Image 1 Highlights: Swine use a reverse order of Ig chain rearrangement compared to humans and mice. Immunoglobulin light chains κ and λ are rearranged and expressed before heavy chains. Unlike mice and humans, many κ+ mature B cells carry λ transcripts in pigs. There are probably two groups of mammals that use different pathway for BCR formation. The reversed order of rearrangement can have evolutional and ontogenetic consequences.