Serotype and genotype diversity of dengue viruses circulating in India: a multi-centre retrospective study involving the Virus Research Diagnostic Laboratory Network in 2018. (October 2021)

• Record Type: Journal Article
• Authors: Alagarasu, K.; Patil, J.A.; Kakade, M.B.; More, A.M.; Yogesh, B.; Newase, P.; Jadhav, S.M.; Parashar, D.; Kaur, H.; Gupta, N.; Vijay, N.; Narayan, J.; Shah, P.S.; Sharma, Dr Ajanta; Jain, Dr Amita; Bhargava, Dr Anudita; Anukumar, Dr B.; M, Dr Ashok; Malhotra, Dr Bharti; Borkakoty, Dr B.J.; Biswas, Dr Debasis; Jaryal, Dr SC; Dhingra, Dr Kanwardeep Singh; Kaveri, Dr K.; Khatri, Dr P.K.; Kumar, Dr Manoj; Nagamani, Dr K.; Dhodapkar, Dr Rahul; Bakshi, Dr Rupinder; Gill, Dr Paramjeet S.; Pati, Dr Sanghamitra; Dutta, Dr Shanta; G, Dr Shantala; Kalawat, Dr Usha; Patel, Dr Disha A.; Vijayachari, Dr P.;
• Is Part Of: International journal of infectious diseases. Volume 111(2021)
• Page Start: 242
• Page End: 252
• Publication Date: 2021-10
• Subjects: Dengue virus serotypes -- Genotypes -- Molecular surveillance -- Virus Research Diagnostic Laboratory (VRDL) Network; Communicable diseases -- Periodicals; Communicable Diseases -- Periodicals; Communicable diseases; Periodicals; Electronic journals; 616.9
• DOI: 10.1016/j.ijid.2021.08.045
• Languages: English
• ISSNs: 1201-9712

Abstract

Highlights: Dengue virus serotypes were tested in 4963 samples collected across India in 2018. DENV-2 was the most prevalent serotype in many states of India. Two genotypes of DENV-1 (I and V) were circulating. Two lineages of DENV-2 genotype IV were circulating. DENV-3 genotype III and DENV-4 genotype-I were circulating. ABSTRACT: Objectives: A retrospective study was undertaken to investigate the circulating dengue virus (DENV) serotypes and genotypes in India in 2018. Methods: In total, 4963 samples referred to virus research diagnostic laboratories (n=21), the Indian Council of Medical Research-National Institute of Virology (ICMR-NIV) and ICMR-NIV field units ( n =2) for diagnosis of dengue in 2018 were tested using a real-time reverse transcription polymerase chain reaction assay for the presence of DENV serotypes. Representative samples were sequenced for the envelope (E) gene. Results: Regional diversity was observed with regard to the dominant circulating serotypes. DENV-2 was found to be the most common serotype in many states. Thrombocytopenia, petechiae and malaise were associated with DENV-2 infection. Phylogenetic analyses of DENV E gene sequences revealed the circulation of genotypes I and V of DENV-1, two lineages of DENV-2 genotype IV, DENV-3 genotype III and DENV-4 genotype I. Conclusions: This study found regional differences in the prevalence of circulating DENV serotypes in India, and provides baseline data for continuous molecular surveillance. Molecular surveillance may have implications for predicting large-scale outbreaks of dengue if regional shifts in the predominantly circulating serotypes and genotypes are detected during the early phase of the dengue season.