Effect of product partition on the synthesis of butyl-β-D-galactoside from Aspergillus oryzae. (November 2021)

• Record Type: Journal Article
• Authors: Muñoz, Kevin; Ahumada, Diego; Arenas, Felipe; Guerrero, Cecilia; Illanes, Andrés; Vera, Carlos
• Is Part Of: Bioresource technology. Volume 340(2021)
• Publication Date: 2021-11
• Subjects: Alkyl-glycoside -- Lactose -- β-galactosidase -- Butyl-β-galactoside; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125697
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Butyl-β-galactoside is 130-fold more soluble in the organic phase than lactose. Higher 1-butanol/aqueous phase ratios increase the reaction yield. Partition of butyl-β-galactoside into the organic phase avoids secondary hydrolysis. Abstract: The effect of donor substrate and products partitioning on the performance of butyl-β-galactoside synthesis with Aspergillus oryzae β-galactosidase was studied. Firstly, the partition coefficient of the donor substrate (lactose) and the reaction products (glucose, galactose and butyl-β-galactoside) were determined in the aqueous and organic phases of the reaction medium. In the temperature range studied (30 to 50 °C), butyl β-galactoside was roughly 130 and 30-fold more soluble in the organic phase than lactose and the monosaccharides, respectively. Afterward, the effect of the 1-butanol/ aqueous phase ratio (α) on the reaction was evaluated in the range from 0.25 to 4. Results show that higher values of α reduce the incidence of secondary hydrolysis by favoring the extraction of butyl-β-galactoside into the organic phase where it is not hydrolyzed, leading to higher yields. Also, major interfacial properties for butyl-β-galactoside were determined at 25 °C.