Design and 3D modeling investigation of a microfluidic electrode array for electrical impedance measurement of single yeast cells. Issue 20 (24th May 2021)

• Record Type: Journal Article
• Authors: Geng, Yangye; Zhu, Zhen; Zhang, Zhao; Xu, Feng; Marchisio, Mario A.; Wang, Zixin; Pan, Dejing; Zhao, Xiangwei; Huang, Qing‐An
• Editors: Hayes, Mark A.
• Is Part Of: Electrophoresis. Volume 42:Issue 20(2021)
• Page Start: 1996
• Page End: 2009
• Publication Date: 2021-05-24
• Subjects: Daughter‐cell dissections -- Electrical impedance spectroscopy -- Microelectrode array -- Saccharomyces cerevisiae -- Single‐cell analysis; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.202100028
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract: High‐resolution microscopic imaging may cause intensive image processing and potential impact of light irradiation on yeast replicative lifespan (RLS). Electrical impedance spectroscopy (EIS) could be alternatively used to perform high‐throughput and label‐free yeast RLS assays. Prior to fabricating EIS‐integrated microfluidic devices for yeast RLS determination, systematic modeling and theoretical investigation are crucial for device design and optimization. Here, we report three‐dimensional (3D) finite‐element modeling and simulations of EIS measurement in a microfluidic single yeast in situ impedance array (SYIIA), which is designed by patterning an electrode matrix underneath a cell‐trapping array. SYIIA was instantiated and modeled as a 5 × 5 sensing array comprising 25 units for cell immobilization, culturing, and time‐lapse EIS recording. Simulations of yeast growing and budding in a sensing unit demonstrated that EIS signals enable the characterization of cell growth and daughter‐cell dissections. In the 5 × 5 sensing array, simulation results indicated that when monitoring a target cell, daughter dissections in its surrounding traps may induce variations of the recorded EIS signals, which could cause mistakes in identifying target daughter‐cell dissections. To eliminate the mis‐identifications, electrode array pitch was optimized. Therefore, the results could conduct the design and optimization of microfluidic electrode‐array‐integrated devices for high‐throughput and accurate yeast RLS assays.