Cloning and characterization of RARs and RXRs from noble scallop Chlamys nobilis, a bivalve rich in carotenoids. (28th June 2021)

• Record Type: Journal Article
• Authors: Tan, Karsoon; Deng, Longhui; Zhang, Hongkuan; Ma, Hongyu; Li, Shengkang; Zheng, Huaiping
• Is Part Of: Aquaculture research. Volume 52:Number 11(2021)
• Page Start: 5283
• Page End: 5296
• Publication Date: 2021-06-28
• Subjects: carotenoid -- Chlamys nobilis -- RAR -- RXR -- SRB‐like‐3 gene interference; Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.15397
• Languages: English
• ISSNs: 1355-557X

Abstract

Abstract: The noble scallop Chlamys nobilis that rich in carotenoids is one of the most popular scallop species for aquaculture. Very little is known whether bivalve SRB ‐ like ‐ 3 gene expression is regulated by retinoic acid receptor (RAR) and/or retinoic X receptor (RXR). In this study, the full‐length cDNA of RAR and RXR genes of C . nobilis was cloned and characterized for the first time, and then, the tissue differential expression of RAR and RXR mRNA in polymorphic C . nobilis was assessed. The roles of RAR and RXR in carotenoid accumulation in C . nobilis were assessed by manipulating the carotenoid content in tissues of C . nobilis via inhibiting the expression of SRB ‐ like ‐ 3 gene. The results revealed that two isoforms of RAR ( cnRARa (1958 bp/434 aa) and cnRARb (1901 bp/415 aa)) and four RXR isoforms ( cnRXRa (1353‐bp/450 aa), cnRXRb (1341‐bp/446 aa), cnRXRc (1298‐bp/436 aa) and cnRXRd (1286‐bp/432 aa)) were cloned and characterized from C . nobilis . The reduction of carotenoid content caused by inhibition of SRB‐like‐3 gene induced the up‐regulation of RAR and RXR genes, suggesting that RAR and RXR genes may play a role in the regulation of carotenoid accumulation in haemolymph of C . nobilis . The findings of this study provide important clues for the regulation mechanism of total carotenoid accumulation in C . nobilis .