Degradation of antimicrobial resistance genes within stockpiled beef cattle feedlot manure. Issue 10 (11th October 2021)

• Record Type: Journal Article
• Authors: Xu, Shanwei; Schwinghamer, Tim; Sura, Srinivas; Cessna, Allan J.; Zvomuya, Francis; Zaheer, Rahat; Larney, Francis J.; McAllister, Tim A.
• Is Part Of: Journal of environmental science and health. Volume 56:Issue 10(2021)
• Page Start: 1093
• Page End: 1106
• Publication Date: 2021-10-11
• Subjects: Feedlot manure -- stockpiling -- chlortetracycline -- sulfamethazine -- tylosin -- quantitative PCR; Environmental engineering -- Periodicals; Environmental sciences -- Periodicals; Ecology -- periodicals; Hazardous Substances -- periodicals; 628
• DOI: 10.1080/10934529.2021.1965416
• Languages: English
• ISSNs: 1093-4529

Abstract

Abstract: Degradation of antimicrobial resistance genes (ARG) in manure from beef cattle administered (kg −1 feed) 44 mg of chlortetracycline (CTC), 44 mg of chlortetracycline plus sulfamethazine (CTCSMZ), 11 mg of tylosin (TYL), or no antimicrobials (Control) was examined. Manure was stockpiled and quantitative PCR (qPCR) was used to assess tetracycline [ tet (C), (L), (M), (W)], erythromycin [ erm (A), (B), (F), (X)], and sulfamethazine [ sul (1), (2)] ARG and 16S rDNA. After 102 d, copies of all ARG decreased by 0.3 to 1.5 log10 copies (g dry matter) −1 . Temperature in the interior of piles averaged ≥ 55 °C for 10 d, except for CTCSMZ, but did not reach 55 °C at pile exteriors. Compared to Control, CTCSMZ increased ( P < 0.05) tet (C), tet (M), tet (W), sul (1), and sul (2) in stockpiled manure. Copies of 16S rDNA remained higher ( P < 0.05) in CTCSMZ than Control for the first 26 d. Levels of most ARG did not differ between the interior and exterior of stockpiles. Our results suggest that stockpiled manure would still introduce ARG to land upon manure application, but at levels lower than if manure was applied fresh.