Quantitative determination of hippuric and m-methylhippuric acids in urine by high-speed liquid chromatography. Issue 4 (November 1977)

• Record Type: Journal Article
• Authors: Matsui, H; Kasao, M; Imamura, S
• Is Part Of: Occupational and environmental medicine. Volume 34:Issue 4(1977)
• Page Start: 310
• Page End: 313
• Publication Date: 1977-11
• Subjects: Medicine, Industrial -- Periodicals; Environmental health -- Periodicals; 616.980305
• DOI: 10.1136/oem.34.4.310
• Languages: English
• ISSNs: 1351-0711

Abstract

Abstract : High-speed liquid chromatography employing an ultraviolet photometric detector has been applied to the simultaneous determination of hippuric and m-methylhippuric acids in urine. Reversed-phase partition chromatography is carried out on a muBondapak C18 column with methanol-water as the eluent system. This method obviates the necessity for isolation or reaction of these acids before assay. The only pretreatment necessary is extraction of sample with ethyl acetate. A linear relationship is obtained between the peak heights and the hippuric or m-methylhippuric acid concentrations. Mean recovery of hippuric and m-methylhippuric acids in urine is 99.8% and 99.3% respectively. The determination of hippuric acid by this method gives lower concentrations in normal urine than does the colorimetric method of Umberger and Fiorese (1963).