Rutin-Zn(II) complex promotes bone formation - A concise assessment in human dental pulp stem cells and zebrafish. (1st November 2021)

• Record Type: Journal Article
• Authors: Vimalraj, Selvaraj; Saravanan, Sekaran; Subramanian, Raghunandhakumar
• Is Part Of: Chemico-biological interactions. Volume 349(2021)
• Publication Date: 2021-11-01
• Subjects: MicroRNA-143-3p -- HDAC7 -- Flavonoid metal complex -- hDPSCs -- Osteoblast differentiation -- Zebrafish; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2021.109674
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: We have assessed the molecular role of Rutin and rutin-Zn(II) complex on osteoblast differentiation and mineralization in human dental pulp cells and zebrafish model. The biocompatibility of the rutin-Zn(II) complex was determined using MTT and chick embryotoxicity assays. Alizarin red staining and ALP measurements were performed to study the osteogenic role of Rutin and rutin-Zn(II) complex at the cellular level in hDPSCs. At molecular level, following rutin and rutin-Zn(II) exposure, the mRNA expression profile of osteoblast markers such Runx2, type 1 col, OC, and ON were investigated. In addition to this, the expression of negative regulators of osteoblast development such Smad7, Smurf1, and HDAC7 waere studied by Real time RT-PCR analysis. The osteogenic role of prepared complex under in vivo was studied by an in-house zebrafish scale model followed by osteoblast differentiation markers expression profiling and Ca:P level measurement by ICP-MS. Rutin and the rutin-Zn(II) complex were found to be non-toxic till 10 μM and increased the expression of osteoblast differentiation marker genes. It also enhanced calcium deposition in both in vitro and in vivo models. Osteogenic property of rutin-Zn(II) in hDPSCs was found be mediated by Smad7, Smurf1, and HDAC7 and enhancing Runx2 expression. Our study warrants the possible use of rutin-Zn(II) as naïve agent or in combination with other bone scaffolding systems/materials for bone tissue engineering applications. Highlights: Rutin-Zn(II) complex promotes osteoblast differentiation in hDPSCs. Smad7, Smurf1 and HDAC7 are downregulated by Rutin-Zn(II) complex. Rutin-Zn(II) complex promotes bone formation in Zebrafish. Ca:P molar ration is increased in Zebrafish scales by Rutin-Zn(II) complex.