Bifunctional utilization of whey powder as a substrate and inducer for β-farnesene production in an engineered Escherichia coli. (December 2021)

• Record Type: Journal Article
• Authors: Ding, Juanjuan; You, Shengping; Ba, Wenyan; Zhang, Hongtao; Chang, Hongxing; Qi, Wei; Su, Rongxin; He, Zhimin
• Is Part Of: Bioresource technology. Volume 341(2021)
• Publication Date: 2021-12
• Subjects: Whey powder -- β-Farnesene -- Escherichia coli -- Bifunctional utilization; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125739
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: The bifunctional utilization of whey powder as substrate and inducer was explored. Whey powder replaced glucose and IPTG to improve β-farnesene titer. Whey powder eliminated highly concentrated glucose repression. The β-farnesene titer was 4.74 g/L using whey powder in the 7 L bioreactor. Abstract: β-Farnesene can replace petroleum products as specialty fuel to solve the global fuel energy crisis, but its production by Escherichia coli ( E.coli ) using glucose and isopropyl β-D-1-thiogalactopyranoside (IPTG) is costly. Hence, we developed a new strategy to produce β-farnesene by engineered E.coli strain F13 with bifunctional utilization of whey powder. The utilization of whey powder as a substrate ensured the growth of the strain F13, while whey powder could also replace IPTG to induce the production of β-farnesene. In shake flasks, β-farnesene production reached 2.41 g/L by the bifunctional utilization of whey powder as a substrate and inducer, 65.1% higher than that with IPTG and glucose. In the 7 L bioreactor, β-farnesene production reached 4.74 g/L using whey powder, which was 197% of that in shake flasks. Therefore, this new strategy might be an attractive route to broaden the applications of whey powder and achieve the economical production of β-farnesene.