DNA/Nano based advanced genetic detection tools for authentication of species: Strategies, prospects and limitations. (October 2021)

• Record Type: Journal Article
• Authors: Khalil, Ibrahim; Hashem, Abu; Nath, Amit R.; Muhd Julkapli, Nurhidayatullaili; Yehye, Wageeh A.; Basirun, Wan Jeffrey
• Is Part Of: Molecular and cellular probes. Volume 59(2021)
• Publication Date: 2021-10
• Subjects: Biosensors -- DNA -- Electrochemical -- Nanomaterials -- Optical -- PCR; Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2021.101758
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: Authentication, detection and quantification of ingredients, and adulterants in food, meat, and meat products are of high importance these days. The conventional techniques for the detection of meat species based on lipid, protein and DNA biomarkers are facing challenges due to the poor selectivity, sensitivity and unsuitability for processed food products or complex food matrices. On the other hand, DNA based molecular techniques and nanoparticle based DNA biosensing strategies are gathering huge attention from the scientific communities, researchers and are considered as one of the best alternatives to the conventional strategies. Though nucleic acid based molecular techniques such as PCR and DNA sequencing are getting greater successes in species detection, they are still facing problems from its point-of-care applications. In this context, nanoparticle based DNA biosensors have gathered successes in some extent but not to a satisfactory stage to mark with. In recent years, many articles have been published in the area of progressive nucleic acid-based technologies, however there are very few review articles on DNA nanobiosensors in food science and technology. In this review, we present the fundamentals of DNA based molecular techniques such as PCR, DNA sequencing and their applications in food science. Moreover, the in-depth discussions of different DNA biosensing strategies or more specifically electrochemical and optical DNA nanobiosensors are presented. In addition, the significance of DNA nanobiosensors over other advanced detection technologies is discussed, focusing on the deficiencies, advantages as well as current challenges to ameliorate with the direction for future development. Highlights: Among the DNA based detection procedures, PCR is the most accepted and current gold standard technique. Various PCR based molecular methods have been discussed as authentication tools. Nanoparticle based electrochemical and optical DNA Biosensors have been emphasized. Nanobiosensors hold great promise for an outburst application in different fields. SERS DNA biosensing is widely employed for the simultaneous detection of single and multiple target analytes.