Prenatal opioid administration induces shared alterations to the maternal and offspring gut microbiome: A preliminary analysis. (1st October 2021)

• Record Type: Journal Article
• Authors: Grecco, Gregory G.; Gao, Yong; Gao, Hongyu; Liu, Yunlong; Atwood, Brady K.
• Is Part Of: Drug and alcohol dependence. Volume 227(2021)
• Publication Date: 2021-10-01
• Subjects: Prenatal -- Microbiome -- Development -- Methadone -- Pregnancy -- Opioid; Drug abuse -- Periodicals; Alcoholism -- Periodicals; 616.86
• DOI: 10.1016/j.drugalcdep.2021.108914
• Languages: English
• ISSNs: 0376-8716

Abstract

Highlights: Maternal opioid use during pregnancy is a growing concern. Maternal methadone treatment disrupts the microbial diversity in dams and offspring. 16 features in dams and 10 features in offspring were differentially abundant. Microbiome changes in dams and offspring demonstrate significant positive correlations. The Lachnospiraceae NK4A136 group may represent a bacterial biomarker of interest associated with opioid exposure. Abstract: Background While many studies have described the impact of prenatal opioid exposure on development, possible mechanisms for how opioids exert developmental impairments remain elusive. Emerging evidence indicates disruptions in the maternal gut microbiome can alter offspring development; however, no studies to date have examined the impact of maternal opioid treatment on maternal-offspring microbiome dysbiosis. Methods A mouse model of prenatal methadone exposure (PME) was employed to assess the impact of maternal opioid treatment on the microbiome of methadone-treated dams (MD) and their offspring. Fecal samples were collected from dams (n = 8 per treatment), one male and one female offspring per dam (n = 8 offspring per sex per treatment) for 16S rRNA sequencing. Results Methadone treatment significantly increased the microbial diversity and led to an expansion in family level bacterial abundance. Correlational analysis revealed significant positive associations between dam and offspring measures of diversity indicating methadone-induced shifts in the microbial communities are shared between dam and offspring. Sixteen features in dams and 10 features in offspring were significantly differentially abundant between treatment groups with many features corresponding to the Lachnospiraceae NK4A136 genus. Of the six features identified as differentially abundant in both MD and PME offspring, all were assigned to the Lachnospiraceae NK4A136 group, and the abundances demonstrated strong positive correlations between dam and offspring. Conclusions These preliminary findings indicate that maternal opioid treatment during pregnancy alters the composition of the maternal microbiome, and this opioid-induced shift is similarly observed in offspring which could contribute to the impaired developmental phenotypes previously described.