Multicentric evaluation of BioFire FilmArray Pneumonia Panel for rapid bacteriological documentation of pneumonia. (September 2021)

• Record Type: Journal Article
• Authors: Gastli, Nabil; Loubinoux, Julien; Daragon, Matthieu; Lavigne, Jean-Philippe; Saint-Sardos, Pierre; Pailhoriès, Hélène; Lemarié, Carole; Benmansour, Hanaa; d'Humières, Camille; Broutin, Lauranne; Dauwalder, Olivier; Levy, Michael; Auger, Gabriel; Kernéis, Solen; Cattoir, Vincent; Alviset, Sophie; Armand-Lefèvre, Laurence; Baldeyrou, Marion; Becker, Agathe; Boibieux, André; Bonacorsi, Stéphane; Burucoa, Christophe; Cambau, Emmanuelle; Casalegno, Jean-Sébastien; Cointe, Aurélie; Cremniter, Julie; Destras, Grégory; Duquaire, Paul; Geslain, Guillaume; Gustave, Claude-Alexandre; Jacquier, Hervé; Kouatchet, Achille; Lecorche, Emmanuel; Lejeune, Manon; Lina, Bruno; Mahieu, Rafaël; Maamar, Adel; Michaud, Anthony; Monnard, Céline; Montravers, Philippe; Neuwirth, Catherine; Péan de Ponfilly, Gauthier; Pichon, Maxime; Plouzeau, Chloé; Poyart, Claire; Quenot, Jean-Pierre; Rzepecki, Vincent; Stéphan, Robin; Timsit, Jean-François; Tran-Dinh, Alexy; Vandenesch, François; Vigier, Emmanuelle;
• Is Part Of: Clinical microbiology and infection. Volume 27:Number 9(2021)
• Page Start: 1308
• Page End: 1314
• Publication Date: 2021-09
• Subjects: Diagnosis -- FilmArray pneumonia panel -- Lower respiratory tract infection -- Multiplex PCR -- Syndromic panel; Medical microbiology -- Periodicals; Diagnostic microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.01
• DOI: 10.1016/j.cmi.2020.11.014
• Languages: English
• ISSNs: 1198-743X

Abstract

Abstract: Objectives: To evaluate performances of the rapid multiplex PCR assay BioFire FilmArray Pneumonia Panel (FA-PP) for detection of bacterial pathogens and antibiotic resistance genes in sputum, endotracheal aspirate (ETA) and bronchoalveolar lavage (BAL) specimens. Methods: This prospective observational study was conducted in 11 French university hospitals (July to December 2018) and assessed performance of FA-PP by comparison with routine conventional methods. Results: A total of 515 respiratory specimens were studied, including 58 sputa, 217 ETA and 240 BAL. The FA-PP detected at least one pathogen in 384 specimens, yielding an overall positivity rate of 74.6% (384/515). Of them, 353 (68.5%) specimens were positive for typical bacteria while eight atypical bacteria and 42 resistance genes were found. While identifying most bacterial pathogens isolated by culture (374/396, 94.4%), the FA-PP detected 294 additional species in 37.7% (194/515) of specimens. The FA-PP demonstrated positive percentage agreement and negative percentage agreement values of 94.4% (95% CI 91.7%–96.5%) and 96.0% (95% CI 95.5%–96.4%), respectively, when compared with culture. Of FA-PP false-negative results, 67.6% (46/68) corresponded to bacterial species not included in the panel. At the same semi-quantification level (in DNA copies/mL for FA-PP versus in CFU/mL for culture), the concordance rate was 43.4% (142/327) for culture-positive specimens with FA-PP reporting higher semi-quantification of ≥1 log10 in 48.6% (159/327) of cases. Interestingly, 90.1% of detected bacteria with ≥10 6 DNA copies/mL grew significantly in culture. Conclusions: FA-PP is a simple and rapid molecular test that could complement routine conventional methods for improvement of diagnosis accuracy of pneumonia.