Effect of autologous adipose tissue-derived mesenchymal stem cells on neovascularization of artificial equine tendon lesions. (November 2014)

• Record Type: Journal Article
• Authors: Conze, Philipp; van Schie, Hans TM; Weeren, René van; Staszyk, Carsten; Conrad, Sabine; Skutella, Thomas; Hopster, Klaus; Rohn, Karl; Stadler, Peter; Geburek, Florian
• Is Part Of: Regenerative medicine. Volume 9:Number 6(2014)
• Page Start: 743
• Page End: 757
• Publication Date: 2014-11
• Subjects: adipose tissue-derived -- color Doppler ultrasonography -- Factor VIII -- mesenchymal stem cells -- neovascularization -- superficial digital flexor tendon -- von Willebrand factor; Cellular therapy -- Periodicals; Stem cells -- Transplantation -- Periodicals; Degeneration (Pathology) -- Treatment -- Periodicals; Regeneration (Biology) -- Periodicals; 611.018
• DOI: 10.2217/rme.14.55
• Languages: English
• ISSNs: 1746-0751

Abstract

Aims: To investigate whether autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) treatment of tendon lesions increases neovascularization during tendon healing.Materials & methods: A standardized surgical model was used to create lesions in both front limb superficial digital flexor tendons (SDFTs) of nine horses. Either AT-MSCs or control substance was injected intralesionally 2 weeks post-surgery. Color Doppler ultrasonography of SDFTs was performed at regular intervals. Horses were euthanized 22 weeks post-treatment and SDFTs were harvested for histology.Results: The color Doppler ultrasonography signal was significantly more extensive at 2 weeks post-treatment and the number of vessels counted on histologic slides was significantly higher at 22 weeks post-treatment in AT-MSC-treated SDFTs.Conclusion: Our findings indicate that AT-MSC treatment has a beneficial effect on neovascularization of healing tendons.