Multipotential stromal cell abundance in cellular bone allograft: comparison with fresh age-matched iliac crest bone and bone marrow aspirate. (2014)

• Record Type: Journal Article
• Authors: Baboolal, Thomas G; Boxall, Sally A; El-Sherbiny, Yasser M; Moseley, Timothy A; Cuthbert, Richard J; Giannoudis, Peter V; McGonagle, Dennis; Jones, Elena
• Is Part Of: Regenerative medicine. Volume 9:Number 5(2014)
• Page Start: 593
• Page End: 607
• Publication Date: 2014
• Subjects: bone marrow -- cellular allograft -- multipotential stromal cells -- osteocytes; Cellular therapy -- Periodicals; Stem cells -- Transplantation -- Periodicals; Degeneration (Pathology) -- Treatment -- Periodicals; Regeneration (Biology) -- Periodicals; 611.018
• DOI: 10.2217/rme.14.17
• Languages: English
• ISSNs: 1746-0751

Abstract

Aim: To enumerate and characterize multipotential stromal cells (MSCs) in a cellular bone allograft and compare with fresh age-matched iliac crest bone and bone marrow (BM) aspirate.Materials & methods: MSC characterization used functional assays, confocal/scanning electron microscopy and whole-genome microarrays. Resident MSCs were enumerated by flow cytometry following enzymatic extraction.Results: Allograft material contained live osteocytes and proliferative bone-lining cells defined as MSCs by phenotypic and functional capacities. Without cultivation/expansion, the allograft displayed an 'osteoinductive' molecular signature and the presence of CD45 - CD271 + CD73 + CD90 + CD105 + MSCs; with a purity over 100-fold that of iliac crest bone. In comparison with BM, MSC numbers enzymatically released from 1 g of cellular allograft were equivalent to approximately 45 ml of BM aspirate.Conclusion: Cellular allograft bone represents a unique nonimmune material rich in MSCs and osteocytes. This osteoinductive graft represents an attractive alternative to autograft bone or composite/synthetic grafts in orthopedics and broader regenerative medicine settings.