Single tumor imaging with multiple antibodies targeting different antigens. (April 2012)

• Record Type: Journal Article
• Authors: Sun, Yujing; Shukla, Girja; Pero, Stephanie C.; Currier, Erika; Sholler, Giselle; Krag, David
• Is Part Of: Biotechniques. Volume 52:Number 4(2012)
• Page Start: 1
• Page End: 3
• Publication Date: 2012-04
• Subjects: combination administration -- antibody -- near-infrared fluorescence imaging -- breast cancer -- xenograft -- mouse; Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000113855
• Languages: English
• ISSNs: 0736-6205

Abstract

Antibodies are important drugs for treating cancer and there is strong rationale for using multiple antibodies to improve outcomes. We labeled two breast cancer binding antibodies, anti-ErbB2 and anti-EpCAM, with infrared fluorescence dyes of different wavelengths and determined their in vivo distribution in a breast cancer xenograft model using a near-infrared (NIR) fluorescence imaging system. Our data show that these two antibodies can be readily assessed simultaneously in mouse xenograft model. This will help guide design of dosing strategies for multiple antibodies and identify potential interaction that could affect pharmacokinetics and possible side effects.