A visualized investigation at the atomic scale of the antitumor effect of magnetic nanomedicine on gastric cancer cells. (July 2014) - Liu, Xiaokang
Aim: Discovering which anticancer drugs attack which organelle(s) of cancer cells is essential and significant, not only for understanding their therapeutic and adverse effects, but also to enable the development of new-generation therapeutics. Here, we show that novel Fe3 O4 –carboxymethyl cellulose–5-fluorouracil (Fe3 O4 –CMC–5FU) nanomedicine can apparently enhance the antitumor effect on gastric cancer cells, and its mechanism of killing the SGC-7901 gastric cancer cells can be directly observed at the atomic scale.Materials & methods: The novel nanomedicine was prepared using the traditional antitumor drug 5FU to chemically bond onto the functionalized Fe3 O4 nanoparticles (Fe3 O4 –CMC–5FU nanomedicine), and then was fed into SGC-7901 gastric cancer cells. The inorganic Fe3 O4 nanoparticles were used to track the distribution and antitumor effect of the nanomedicine within individual SGC-7901 gastric cancer cells.Results & discussion: Atomic-level observation and tracking the elemental distribution inside individual cells proved that the magnetic nanomedicine killed the gastric cells mainly by attacking their mitochondria. The enhanced therapeutic efficacy derives from the localized high concentration and poor mobility of the aggregated Fe3 O4 –CMC–5FU nanomedicine in the cytoplasm.Conclusion: A brand new mechanism of Fe3 O4 –CMC–5FU nanomedicine killing SGC-7901 gastric cancer cells by attacking their mitochondria was discovered, which is different from the classical mechanism utilized by traditional medicine 5FU, which kills gastric cancer cells by damaging their DNA. Our work might provide a partial solution in nanomedicines or even modern anticancer medicine for the visualized investigation of their antitumor effect. Original submitted 25 January 2013; Revised submitted 10 May 2013