Variations of the corona HDL:albumin ratio determine distinct effects of amorphous SiO2 nanoparticles on monocytes and macrophages in serum. (2014)

• Record Type: Journal Article
• Authors: Fedeli, Chiara; Segat, Daniela; Tavano, Regina; De Franceschi, Giorgia; de Laureto, Patrizia Polverino; Lubian, Elisa; Selvestrel, Francesco; Mancin, Fabrizio; Papini, Emanuele
• Is Part Of: Nanomedicine. Volume 9:Number 16(2014)
• Page Start: 2481
• Page End: 2497
• Publication Date: 2014
• Subjects: amorphous silica nanoparticle -- HDL -- macrophage -- monocyte -- nanoparticle protein corona -- pyroptosis; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.14.22
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: We investigated monocyte and macrophage death and cytokine production induced by amorphous silica nanoparticles (SiO2 -NPs) to clarify the role of defined serum corona proteins.Materials & methods: The cytotoxic proinflammatory effects of SiO2 -NPs on human monocytes and macrophages were characterized in no serum, in fetal calf serum and in the presence of purified corona proteins.Results: In no serum and in fetal calf serum above approximately 75 µg/ml, SiO2 -NPs lysed monocytes and macrophages by plasma membrane damage (necrosis). In fetal calf serum below approximately 75 µg/ml, SiO2 -NPs triggered an endolysosomal acidification and caspase-1-dependent monocyte death (pyroptosis). The corona high-density lipoproteins:albumin ratio accounted for the features of the SiO2 -NPs in serum.Discussion: Corona high-density lipoproteins are a major determinant of the differential cytotoxic action of SiO2 -NPs on monocytes and macrophages. Original submitted 15 October 2013; Revised submitted 20 January 2014