Cellular magnetic resonance with iron oxide nanoparticles: long-term persistence of SPIO signal in the CNS after transplanted cell death. (2014)

• Record Type: Journal Article
• Authors: Cianciaruso, Chiara; Pagani, Antonella; Martelli, Cristina; Bacigaluppi, Marco; Leonardo Squadrito, Mario; Lo Dico, Alessia; De Palma, Michele; Furlan, Roberto; Lucignani, Giovanni; Falini, Andrea; Biffi, Alessandra; Ottobrini, Luisa; Salvatore Politi, Letterio
• Is Part Of: Nanomedicine. Volume 9:Number 10(2014)
• Page Start: 1457
• Page End: 1474
• Publication Date: 2014
• Subjects: cell death -- cellular MRI -- diphtheria toxin -- microglial cell -- specificity -- superparamagnetic iron oxide; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.14.84
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To study the specificity of cellular MRI based on superparamagnetic iron oxide particles (SPIOs), especially within the CNS.Materials & methods: A microglial cell line was engineered for the expression of a suicide gene, the receptor of diphtheria toxin (DT), and two reporter genes, green fluorescent protein and luciferase, in order to induce, in a controlled manner, cell death and test it through bioluminescence. SPIO-labeled DT-sensitive and control DT-insensitive cells were transplanted into the brains of mice, which underwent serial MRI and bioluminescence studies before and up to 90 days after DT-induced cell death.Results: No variations in SPIO signal voids were detected along longitudinal monitoring in brain hemispheres transplanted with DT-sensitive cells. Ex vivo analyses showed persistence of iron nanoparticle deposits at transplantation sites.Conclusion: Due to the long-term persistence of signal after transplanted cell death, caution is advised when SPIOs are employed for cell tracking. Original submitted 27 January 2014; Revised submitted 18 April 2014