Microarray analysis revealed markedly differential miRNA expression profiles in cervical intraepithelial neoplasias and invasive squamous cell carcinoma. (2014)

• Record Type: Journal Article
• Authors: Liang, Shuang; Tian, Tian; Liu, Xubin; Shi, Huijuan; Tang, Cuilan; Yang, Shicong; Wang, Liantang; Chen, Shangwu; Yu, Li
• Is Part Of: Future oncology. Volume 10:Number 13(2014)
• Page Start: 2023
• Page End: 2032
• Publication Date: 2014
• Subjects: cervical cancer -- cervical intraepithelial neoplasias -- CIN -- FFPE tissue -- formalin-fixed, paraffin-embedded tissue -- microarray -- microRNAs -- miRNAs; Oncology -- Periodicals; 616.99405
• DOI: 10.2217/fon.14.38
• Languages: English
• ISSNs: 1479-6694

Abstract

ABSTRACT Aims: To investigate the alterations in miRNA expression during the progression of dysplasia in cervical epithelium.Methods: A global miRNA expression profile of normal cervical squamous epithelium (Normal), cervical intraepithelial neoplasia (CIN) 3 and invasive squamous cell carcinoma (ISCC) was produced using the seventh generation of the miRCURY™ LNA microRNA Array (Exiqon, Vedbaek, Denmark). The reliability of miRNA arrays was verified by reverse transcription PCR.Results: Normal, CIN 3 and ISCC showed distinct miRNA expression profiles. The differentially expressed miRNAs in ISCC versus CIN 3 clearly differed from that in CIN 3 versus Normal. Compared with ISCC versus Normal, more identical miRNAs were found in ISCC versus CIN 3 than in CIN 3 versus Normal.Conclusion: A particular set of miRNAs was associated with the progression of normal cervical epithelium to CIN 3 and CIN 3 to ISCC. The miRNA profile changed more noticeably in the progression of CIN to ISCC than normal cervical epithelium to CIN.