Immunoreactivity of synthetic peptides derived from proteins of Cryptococcus gattii. (July 2014)

• Record Type: Journal Article
• Authors: de Serpa Brandão, Rafael Melo Santos; Soares Martins, Liline Maria; de Andrade, Hélida Monteiro; Faria, Angélica Rosa; Soares Leal, Maria José; da Silva, Adalberto Socorro; Wanke, Bodo; dos Santos Lazéra, Márcia; Vainstein, Marilene Henning; Mendes, Rinaldo Poncio; Moris, Daniela Vanessa; de Souza Cavalcante, Ricardo; do Monte, Semiramis Jamil Hadad
• Is Part Of: Future microbiology. Volume 9:Number 7(2014:Jul.)
• Page Start: 871
• Page End: 878
• Publication Date: 2014-07
• Subjects: antigens -- cryptococcosis -- diagnosis -- ELISA -- synthetic peptides; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb.14.49
• Languages: English
• ISSNs: 1746-0913

Abstract

ABSTRACT: : Aim: To determine the immunoreactivity of synthetic Cryptococcus -derived peptides.Materials & methods: A total of 63 B-cell epitopes from previously identified Cryptococcus gattii immunoreactive proteins were synthesized and evaluated as antigens in ELISAs. The peptides were first evaluated for their ability to react against sera from immunocompetent subjects carrying cryptococcal meningitis. Peptides that yielded high sensitivity and specificity in the first test were then retested with sera from individuals with other fungal pathologies for cross-reactivity determination.Results: Six of 63 synthetic peptides were recognized by antibodies in immunoassays, with a specificity of 100%, sensitivity of 78% and low cross-reactivity.Conclusion: We successfully determined the immunoreactivity of selected synthetic peptides of C. gattii derived proteins.