Next-generation protein analysis in the pathology department. Issue 1 (15th July 2019)

• Record Type: Journal Article
• Authors: Ahmed, Melek; Broeckx, Glenn; Baggerman, Geert; Schildermans, Karin; Pauwels, Patrick; Van Craenenbroeck, Amaryllis H; Dendooven, Amelie
• Is Part Of: Journal of clinical pathology. Volume 73:Issue 1(2020)
• Page Start: 1
• Page End: 6
• Publication Date: 2019-07-15
• Subjects: proteomics -- MALDI IMS -- mass spectrometry -- pathology; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jclinpath-2019-205864
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Traditionally, immunohistochemistry (IHC) is used by pathologists to localise specific proteins or peptides in tissue slides. In the era of personalised medicine, however, molecular tissue analysis becomes indispensable for correct diagnosis, prognosis and therapeutic decision, not only on the DNA or mRNA level but also on the protein level. Combining molecular information with imaging presents many advantages. Therefore, matrix-assisted laser desorption/ionisation imaging mass spectrometry (MALDI IMS) is a promising technique to be added to the armamentarium of the pathologist. Here, we focus on the workflow, advantages and drawbacks of both MALDI IMS and IHC. We also briefly discuss a few other protein imaging modalities and give examples of applications.