Aptamer selection by high-throughput sequencing and informatic analysis. (December 2011)

• Record Type: Journal Article
• Authors: Hoon, Shawn; Zhou, Bin; Janda, Kim D.; Brenner, Sydney; Scolnick, Jonathan
• Is Part Of: Biotechniques. Volume 51:Number 6(2011)
• Page Start: 413
• Page End: 416
• Publication Date: 2011-12
• Subjects: aptamer -- high throughput sequencing -- informatics; Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000113786
• Languages: English
• ISSNs: 0736-6205

Abstract

Traditional methods for selecting aptamers require multiple rounds of selection and optimization in order to identify aptamers that bind with high affinity to their targets. Here we describe an assay that requires only one round of positive selection followed by high-throughput DNA sequencing and informatic analysis in order to select high-affinity aptamers. The assay is flexible, requires less hands on time, and can be used by laboratories with minimal expertise in aptamer biology to quickly select high-affinity aptamers to a target of interest. This assay has been utilized to successfully identify aptamers that bind to thrombin with dissociation constants in the nanomolar range.