Validation of a commercial human ELISA to measure hyaluronic acid concentration in feline plasma. (January 2022)

• Record Type: Journal Article
• Authors: Shaw, Kaela E.; Bersenas, Alexa M.; Bateman, Shane W.; Blois, Shauna L.; Wood, R. Darren
• Is Part Of: Journal of veterinary diagnostic investigation. Volume 34:Number 1(2022)
• Page Start: 86
• Page End: 89
• Publication Date: 2022-01
• Subjects: ELISA -- endothelial glycocalyx -- feline -- hyaluronic acid -- validation; Veterinary medicine -- Diagnosis -- Periodicals; 636.0896075
• DOI: 10.1177/10406387211044718
• Languages: English
• ISSNs: 1040-6387

Abstract

Our goal was to validate a human hyaluronic acid (HA) ELISA (Hyaluronic acid plus ELISA; TECOmedical Group) for use in feline plasma. Plasma from 5 healthy cats and 5 critically ill cats was used for validation of the assay. Validation methods performed included intra- and inter-assay variability, spike-and-recovery, and dilutional linearity. All measurements were performed in duplicate. The precision study revealed good intra-assay CV of 7.4–8.9%; inter-assay CV was 3.4–4.2%. Extraction efficiency via spiking tests yielded mean recovery of 89.6%. The assay met criteria for acceptable linearity using 3 serial dilutions. Our results demonstrate that this commercial HA ELISA had acceptable analytical performance using feline plasma and could be a useful tool in the veterinary clinical research setting.