Differing reflections of paediatric classical Hodgkin's lymphoma on local and distant immunological microenvironments: a flow cytometric study. Issue 3 (21st September 2019)

• Record Type: Journal Article
• Authors: Savaşan, Süreyya; AlQanber, Batool; Henry, Meret; Buck, Steven; Gadgeel, Manisha
• Is Part Of: Journal of clinical pathology. Volume 73:Issue 3(2020)
• Page Start: 176
• Page End: 179
• Publication Date: 2019-09-21
• Subjects: Hodgkin lymphoma -- immunological microenvironment -- lymph node -- bone marrow -- flow cytometry; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jclinpath-2019-205967
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Aims: To compare immunological microenvironments in local and distant lymphoid tissues in Hodgkin's lymphoma (HL) in children. Methods: We have analysed diagnostic bone marrow (BM) samples in 22 and corresponding involved lymph node (LN) in eight and peripheral blood (PB) in eight cases of HL by flow cytometry and sought correlations with clinical features retrospectively. Results: While there were significant differences in lymphocyte compositions of BM and LN tissues, the distribution of lymphocyte subsets mimicked each other in BM and PB. CD8-positive cytotoxic T cells predominate the bone marrow in contrast to CD4-positive helper T cells in LN tissue with corresponding CD4/CD8 ratios (0.85 and 5.3, respectively; p=0.002). Additionally, T-large granular lymphocytes population was much higher in BM in comparison to LN tissue (10.5% vs 4.5%; p=0.036). Conclusions: Local immunological microenvironment appears to be highly influenced by HL tumour cells and distant site lymphocyte composition reflects immune response to control the neoplastic process.