A Robust Auxin Response Network Controls Embryo and Suspensor Development through a Basic Helix Loop Helix Transcriptional Module. Issue 1 (9th January 2019)

• Record Type: Journal Article
• Authors: Radoeva, Tatyana; Lokerse, Annemarie S.; Llavata-Peris, Cristina I.; Wendrich, Jos R.; Xiang, Daoquan; Liao, Che-Yang; Vlaar, Lieke; Boekschoten, Mark; Hooiveld, Guido; Datla, Raju; Weijers, Dolf
• Is Part Of: The Plant Cell. Volume 31:Issue 1(2019)
• Page Start: 52
• Page End: 67
• Publication Date: 2019-01-09
• DOI: 10.1105/tpc.18.00518
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract : A robust auxin network controls reprogramming toward embryogenesis in suspensor cells via a basic Helix-Loop-Helix transcriptional module, a process involving large transcriptomic changes before anatomical changes. Abstract: Land plants reproduce sexually by developing an embryo from a fertilized egg cell. However, embryos can also be formed from other cell types in many plant species. Thus, a key question is how embryo identity in plants is controlled, and how this process is modified during nonzygotic embryogenesis. The Arabidopsis ( Arabidopsis thaliana ) zygote divides to produce an embryonic lineage and an extra-embryonic suspensor. Yet, normally quiescent suspensor cells can develop a second embryo when the initial embryo is damaged, or when response to the signaling molecule auxin is locally blocked. Here we used auxin-dependent suspensor embryogenesis as a model to determine transcriptome changes during embryonic reprogramming. We found that reprogramming is complex and accompanied by large transcriptomic changes before anatomical changes. This analysis revealed a strong enrichment for genes encoding components of auxin homeostasis and response among misregulated genes. Strikingly, deregulation among multiple auxin-related gene families converged upon the re-establishment of cellular auxin levels or response. This finding points to a remarkable degree of feedback regulation to create resilience in the auxin response during embryo development. Starting from the transcriptome of auxin-deregulated embryos, we identified an auxin-dependent basic Helix Loop Helix transcription factor network that mediates the activity of this hormone in suppressing embryo development from the suspensor.