Morphological and molecular characterization of Spirocerca lupi (Nematoda: Spirocercidae) from domestic dogs in Vietnam with reference to Spirocerca vulpis. (October 2021)

• Record Type: Journal Article
• Authors: Hoa, Le Thi Khanh; Hien, Hoang Van; Phuc, Phan Thi Hong; Lan, Nguyen Thi Kim; Doanh, Pham Ngoc
• Is Part Of: Parasitology international. Volume 84(2021)
• Publication Date: 2021-10
• Subjects: Molecular analysis -- Morphology -- Spirocerca lupi -- Spirocerca vulpis; Parasitology -- Periodicals; Parasites -- Periodicals; Parasitic Diseases -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; 571.99905
• DOI: 10.1016/j.parint.2021.102381
• Languages: English
• ISSNs: 1383-5769

Abstract

Abstract: Spirocerca lupi is a common parasitic nematode associated with esophageal cancer of canids. Recent surveys have revealed an increasing number of canids infected with Spirocerca spp. in Africa, the Americas, Europe and Western Asia, and described a new species, Spirocerca vulpis, from red foxes ( Vulpes vulpes ). However, in Southeast Asia, research on Spirocerca spp. is scarce. Therefore, the aim of this study is to explore Spirocerca infection in domestic dogs in Vietnam and to identify the Spirocerca species by analyzing their morphometric and molecular data. We found Spirocerca spp. specimens in 51 (17.7%) out of 287 dogs examined with the intensity of infection ranging from one to 29 nematodes per dog. These nematodes were morphologically and molecularly identified as S. lupi . For morphology, the presence/absence of teeth, the ratio of glandular to muscular esophagus lengths, and the position of the vulva opening of S. lupi and S. vulpis varied between reports, suggesting caution in identification of Spirocerca species based exclusively on morphological characteristics. The molecular analysis based on a partial cox1 sequence revealed that S. lupi from Vietnam is genetically close to those from India and China, but far different from those of Israel, South Africa, Peru and Hungary. Given high genetic and morphological variations, more extensive surveys on Spirocerca spp. from various mammalian hosts at a greater scale are necessary to elucidate the divergence of this nematode. Graphical abstract: Unlabelled Image Highlights: Spirocerca nematodes were found from 51 (17.7%) domestic dogs in Northern Vietnam. Spirocerca spp. from Vietnam were morphologically and molecularly identifed as S. lupi . S. lupi from Vietnam was genetically closest to Indian and Chinese specimens. Morphological characteristics of Spirocerca spp. vary among reports. More precise features for differentiation among Spirocerca species are necessary.