Penaeicolahalotolerans gen. nov., sp. nov., a novel bacterium of the family Cyclobacteriaceae isolated from a shrimp pond. Issue 10 (8th October 2021)

• Record Type: Journal Article
• Authors: Ali, Sardar; Xie, Jianmin; Chen, Yuerong; Cai, Runlin; Juventus, Aweya Jude; Hu, Zhong; Zhang, Yueling; Wang, Hui
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 10(2021)
• Publication Date: 2021-10-08
• Subjects: antimicrobial resistant genes -- average amino acid identity -- Cyclobacteriaceae -- percentage of conserved proteins -- xenobiotics biodegradation; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005047
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterium (designated as LMIT005 T ) was isolated from shrimp ponds in Shantou, China. The new isolate was characterized taxonomically using a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain LMIT005 T was found to be affiliated with the family Cyclobacteriaceae of the order Cytophagales while appearing as a distinct lineage. The 16S rRNA gene sequence similarity between strain LMIT005 T and Algoriphagus yeomjeoni KCTC 12309 T, the closest type strain in the family, was 91.3 %. Strain LMIT005 T grew optimally at 25 °C, pH 7 and in the presence of 2.0 % (w/v) NaCl. The DNA G+C content (data from genome sequence) was 40.5 mol%. Compared with reference strain A. yeomjeoni KCTC 12309 T, the average nucleotide identity (ANI) of LMIT005 T was 70 %. The sole respiratory quinone of LMIT005 T was menaquinone (MK-7), and the major fatty acids were summed feature 3 (C16 : 1 ω6c / C16 : 1 ω7c). The polar lipids of strain LMIT005 T were mainly composed of phosphatidylethanolamine, phosphatidylcholine, two unidentified amino lipids, two unidentified lipids, one unidentified glycolipid and one unidentified phospholipid. The draft genome of strain LMIT005 T comprised 3 089 781 bp (3.09 Mb) nucleotides and 2773 genes. Antimicrobial resistant-related genes ( blal, mexA, and mexb ) were annotated in the genome of strain LMIT005 T, which indicated that it might be able to resist β-lactam antibiotics. This was further verified by antimicrobial resistant test. Given its distinct genomic, morphological, and physiological differences from previously described type strains, strain LMIT005 T is proposed as a representative of a novel genus of the family Cyclobacteriaceae, with the name Penaeicola halotolerans gen. nov., sp. nov. The type strain is LMIT005 T (=KCTC 82616 T =CICC 25047 T ).