Combined impacts of diclofenac and divalent copper on the nitrogen removal, bacterial activity and community from a sequencing batch reactor. (October 2021)

• Record Type: Journal Article
• Authors: Yang, Huan; Wang, Zichao; Yuan, Shengyu; Wang, Yueyue; Song, Youtao; Bu, Naishun; Wang, Lin; Zhang, Lu
• Is Part Of: Journal of water process engineering. Volume 43(2021)
• Publication Date: 2021-10
• Subjects: Diclofenac -- Divalent copper -- Combined impacts -- Nitrogen removal -- Enzyme activity; Water-supply engineering -- Periodicals; Saline water conversion -- Periodicals; Seawater -- Distillation -- Periodicals; Sanitary engineering -- Periodicals; Sewage -- Purification -- Periodicals; 627
• DOI: 10.1016/j.jwpe.2021.102212
• Languages: English
• ISSNs: 2214-7144

Abstract

Abstract: Combined impacts of diclofenac (DCF) and divalent copper (Cu 2+ ) on the nitrogen removal, enzyme activity and bacterial community from a sequencing batch reactor were examined. DCF and/or Cu 2+ in the influent rendered the decrease of ammonia nitrogen and chemical oxygen demand elimination rates. The combined inhibition impacts of Cu 2+ and DCF on ammonia monooxygenase (AMO), nitrite oxidoreductase (NOR), nitrite reductase (NIR) and nitrate reductase (NAR) contents exhibited synergetic, and the AMO and NOR activities reduced levels were lower than NAR and NIR as the mixed Cu 2+ and DCF concentration increment. The decreased degrees of AMO activities were higher than NOR at different mixed Cu 2+ and DCF concentrations. The contents of reactive oxygen species and lactate dehydrogenase at the mixed Cu 2+ and DCF stress were higher than those at the alone Cu 2+ and DCF stress, and they increased as the mixed Cu 2+ and DCF concentration increase. In extracellular polymeric substances (EPS), the content of protein (PN) was more easily affected by Cu 2+ and mixed Cu 2+ /DCF than polysaccharide (PS), while PN and PS were both sensitive to the stress of DCF. Compared to seeding sludge, the denitrifying bacteria and nitrifying bacteria relative abundance ratio was decreased at the stress of alone Cu 2+ and DCF, while the ratio was increased as the mixed Cu 2+ and DCF concentration increment. Graphical abstract: Unlabelled Image Highlights: Combined effects of Cu 2+ and DCF on AMO, NOR, NAR and NIR were synergetic. AMO and NAR were more easily inhibited by mixed Cu 2+ /DCF than NOR and NIR. In EPS, PN was more sensitive to Cu 2+ and mixed Cu 2+ /DCF than PS. Denitrifiers had a greater tolerance to mixed Cu 2+ /DCF than nitrifiers.