Interleukin enhancer binding factor 2 (IEBF 2) was involved in the regulation of the antibacterial immune reactions in fresh water crayfish, Procambarus clarkii. (January 2022)

• Record Type: Journal Article
• Authors: Yang, Bingbing; Lin, Sihan; Li, Bo; Wei, Zhe; Li, Qianqian; Shen, Xiuli; Du, Zhiqiang
• Is Part Of: Developmental and comparative immunology. Volume 126(2022)
• Publication Date: 2022-01
• Subjects: Procambarus clarkii -- Interleukin enhancer binding factor gene -- Bacterial challenge -- RNAi assay -- Innate immune; IEBF interleukin enhancer binding factor -- CSF colony stimulating factor -- TNF tumor necrosis factor -- IL interleukin -- IL-1 interleukin-1 -- IL-2 Interleukin-2 -- IEBF 2 interleukin enhancer binding factor 2 -- NF45 nuclear factor 45 -- NLRP3, NACHT LRR and PYD domains-containing protein 3 -- BLAST Web-based Basic Local Alignment Search Tool -- NJ Neighbor Joining -- qRT-PCR quantitative real-time PCR -- dsRNA double strand RNA -- dsIEBF 2 IEBF 2 dsRNA -- dsGFP GFP dsRNA -- ORF open reading frame -- DZF domain associated with zinc fingers -- NF90 nuclear factor 90 -- rCDV recombinant canine distemper virus; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2021.104226
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Although interleukin and interleukin analogues which play important immunomodulatory roles in mammals have not yet been reported in invertebrates, interleukin enhancer binding factor (IEBF) which acts as a transcription factor has been recently studied in several crustaceans and it may be involved in innate immune defence against pathogens. In this study, an IEBF 2 homologue was identified in the fresh water crayfish, Procambarus clarkii . The significantly changed expression levels of Pc - iebf 2 after bacterial challenge revealed the possibility of its participation in defence against bacterial infection. The results of an RNAi assay showed that the crayfish survival rate was obviously decreased after dsIEBF 2 injection, compared with the control groups. And S. aureus proliferation was obviously enhanced at 24 and 48 h post bacterial injection, when Pc - iebf 2 was knocked down. The possible molecular mechanisms for the innate immune regulation functions of Pc -IEBF 2 were also investigated. We speculated that Pc -IEBF 2 plays an important role in defending against bacterial infection in crayfish. It could regulate some innate immune responses by affecting the Toll signalling pathway, melanisation, and cell apoptosis. Highlights: Crayfish survival rate was decreased after Pc - iebf 2 was knocked down. Pc -IEBF 2 affected the production of AMPs and the trigger of melanisation. After Pc - iebf 2 was silenced, proapoptosis-associated genes were suppressed.