Identification of wnt2 in the pearl mussel Hyriopsis cumingii and its role in innate immunity and gonadal development. Issue 118 (November 2021)

• Record Type: Journal Article
• Authors: Wang, Yayu; Chen, Ya; Cao, Mulian; Wang, Xiaoqiang; Wang, Guiling; Li, Jiale
• Is Part Of: Fish & shellfish immunology. Issue 118(2021)
• Page Start: 85
• Page End: 93
• Publication Date: 2021-11
• Subjects: wnt2 -- Hyriopsis cumingii -- Immune stimulation -- Innate immunity -- Wnt signaling pathway -- Gonadal development -- miR-1988b-5p; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2021.08.022
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Wnt2 is a significant factor in the Wnt signaling pathway, which is associated with a variety of physiological activities, including inflammatory response, cell apoptosis, reproductive system development, and cell differentiation. Hyriopsis cumingii is the main pearl breeding mussel in China. However, the role of wnt2 in this species remains unclear. In this study, wnt2 from H. cumingii was cloned and identified. The full-length cDNA of wnt2 is 1524 bp, containing a 963 bp open reading frame (ORF), encoding 320 amino acid residues. The tissue distribution of H. cumingii indicated that wnt2 was predominantly highly expressed in the ovary and gill. And the expression profile after Aeromonas hydrophila or LPS injection indicated that wnt2 was up-regulated in gill, suggesting its role in the innate immune response. The expression of wnt2 was high at 4-month-old of early gonadal development and throughout ovarian development. In situ hybridization (ISH) showed significant hybridization signals on the gills and mature eggs of female gonads. In addition, miR-1988b-5p was found to negatively regulate wnt2 to affect the expression of key genes ( frizzled-5, ctnnb1, and tcf7l ) in the Wnt signaling pathway. Thus, these findings suggest a key role for wnt2 in immune regulation and gonadal development in H. cumingii . Highlights: wnt2 of Hyriopsis cumingii was identified and characterized. The highest wnt2 expression was observed in ovary and gill. wnt2 is up-regulated in gill following Aeromonas hydrophila /LPS exposure. wnt2 is involved in immune regulation and gonadal development of H.cumingii. MiR-1988b-5p affects Wnt signaling pathway genes expression by targeting wnt2.